452 Housekeeping gene selection and validation for qPCR analysis of psoriasis biopsies
نویسندگان
چکیده
منابع مشابه
Identification and Validation of Housekeeping Genes for Gene Expression Analysis of Cancer Stem Cells
The characterization of cancer stem cell (CSC) subpopulation, through the comparison of the gene expression signature in respect to the native cancer cells, is particularly important for the identification of novel and more effective anticancer strategies. However, CSC have peculiar characteristics in terms of adhesion, growth, and metabolism that possibly implies a different modulation of the ...
متن کاملValidation of the comparative quantification method of real-time PCR analysis and a cautionary tale of housekeeping gene selection
Richard D. McCurdy*, John J. McGrath, Alan Mackay-Sim Eskitis Institute for Cell and Molecular Therapies, and School of Biomedical and Biomolecular Science, Griffith University, Brisbane, QLD 4111, Australia Queensland Centre for Mental Health Research, The Park Centre for Mental Health, Wacol QLD 4076, Australia Department of Psychiatry, University of Queensland, St Lucia QLD 4072, Australia _...
متن کاملReference Gene Selection for qPCR Analysis in Tomato-Bipartite Begomovirus Interaction and Validation in Additional Tomato-Virus Pathosystems
Quantitative Polymerase Chain Reaction (qPCR) is currently the most sensitive technique used for absolute and relative quantification of a target gene transcript, requiring the use of appropriated reference genes for data normalization. To accurately estimate the relative expression of target tomato (Solanum lycopersicum L.) genes responsive to several virus species in reverse transcription qPC...
متن کاملRubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea
The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis-phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by ...
متن کاملReference Gene Selection for RT-qPCR Analysis of Flower Development in Chrysanthemum morifolium and Chrysanthemum lavandulifolium
Quantitative real-time PCR (qPCR) is a popular and powerful tool used to understand the molecular mechanisms of flower development. However, the accuracy of this approach depends on the stability of reference genes. The capitulum of chrysanthemums is very special, which is consisting of ray florets and disc florets. There are obvious differences between the two types of florets in symmetry, gen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2017
ISSN: 0022-202X
DOI: 10.1016/j.jid.2017.07.648